Features of metabolic changes in the liver in experimental animals under chronic exposure to acrylamide and against the background of its preventive correction

Abstract

Introduction. Acrylamide used industrially is also widely known to be a toxic compound produced during high temperature cooking. Given the danger of its entry into the body, it is important to continue fundamental research to study the mechanism of its toxicity and search for effective ways to correct the disorders caused by it. 
 The purpose of the research is to study the features of metabolic changes in the liver of rats under conditions of chronic exposure to acrylamide and evaluate the effectiveness of their preventive correction with oxymethyluracil complex compounds.
 Materials and methods. Studies were performed on sixty white outbred male rats weighing 180–200 g. Acrylamide was administered intragastrically at a dose of 5 mg/kg of body weight for 90 days. Correction of possible disorders was carried out 1 hour before the administration of the toxicant with complex compounds of oxymethyluracil with ascorbic acid (MG-1), with sodium succinate (MG-2) and acetylcysteine (MG-10). Biochemical parameters were studied after 
 45 and 90 days of the experiment.
 Results. Studies have shown long-term intake of acrylamide at a dose of 5 mg/kg of body weight to lead to metabolic disorders. A statistically significant increase in the activity of the enzymes aspartate aminotransferase (AST), alanine aminotransferase (ALT), alkaline phosphatase (AP), and superoxide dismutase (SOD) was found. Statistically significant differences in the level of activity of ALT, alkaline phosphatase and SOD for the period of the experiment of 45 days compared with the positive control group were established in the group receiving the MG-10 preparation. After 90 days, in the group of animals treated with the MG-2 preparation, the activity of ALT and alkaline phosphatase decreased significantly. The introduction of the drug MG-10 had a statistically significant effect on ALT activity, reducing it to the level in the negative control group of animals treated with distilled water.
 Limitations of the study are related to the correction with complex compounds of oxymethyluracil carried out only in a prophylactic regimen. For the final judgment on their protective effectiveness, it is necessary to introduce them in the accompanying and restorative regimes, as well as to study other biochemical parameters when exposed to acrylamide.
 Conclusion. The best protective effect at a period of 45 days was shown by a complex compound of oxymethyluracil with acetylcysteine, at a period of 90 days – 
 a complex compound of oxymethyluracil with sodium succinate.