Abstract
In this study, factors such as pH, temperature, carbon and nitrogen sources that affect mycelial growth of Hydnum repandum, a medicinal mushroom, were investigated. Different inoculum media for vegetative inoculum production were also examined. The best suitable pH for mycelial growth was found to be 5.5. Among constant temperatures, the best mycelial growth was obtained at 20 and 25°C. The mycelial growth drastically decreased at 15°C, and no mycelia were obtained at 30°C. Glucose and mannitol were found to be the most suitable carbon sources. Ca(NO3)2 as a nitrogen source gave the best results for mycelial growth. The poorest mycelial growth was noted in sucrose and xylose as carbon sources and in NH4NO3 and (NH4)2HPO4 as nitrogen sources. Peat and peat: vermiculite mixtures (1:4, 1:6, 1:8 and 1:10, v:v) were the best media to use in producing the vegetative inoculum of H. repandum.
Highlights
Ectomycorrhizal and medicinal mushroom, which belongs to the class Basidiomycetes, the order Cantharellales and the family Hydnaceae (Phillips, 1994)
H. repandum is an important source of income and valuable human food for rural population due to its nutritional and medical properties
The mycelial culture was maintained on Potato Dextrose Agar (PDA) at 25°C in complete darkness, and the cultures were stored at 4°C
Summary
Hydnum repandum (L. ex Fr.) S.F. Gray is an edible, ectomycorrhizal and medicinal mushroom, which belongs to the class Basidiomycetes, the order Cantharellales and the family Hydnaceae (Phillips, 1994). Ectomycorrhizal and medicinal mushroom, which belongs to the class Basidiomycetes, the order Cantharellales and the family Hydnaceae (Phillips, 1994) It is commonly known as the Wood Hedgehog or Hedgehog mushroom. H. repandum is an important source of income and valuable human food for rural population due to its nutritional and medical properties. Album displayed potent cytotoxic activity against various tumour cells (Takahashi et al, 1992). Murcia et al (2002) stated that H. repandum has antioxidant activity. It is reported that H. repandum is an important nutrient for diet (Alonso et al, 2003)
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More From: African Journal of Traditional, Complementary and Alternative Medicines
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