Abstract
Four glycolipids have been isolated from three fractions of pig blood. The glycolipids were presumably cerebroside, diglycosyl ceramide, triglycosyl ceramide, and globoside. The blood fractions were erythrocytes and plasma high and low density lipoproteins. Fatty acid distributions were determined for each glycolipid as a means to assist in identifying relationships among the several glycolipids. Normal fatty acids predominated in all glycolipids except the globosides from erythrocytes in which the amount of hydroxy acids was slightly greater than the amount of normal acids. Hydroxy acids appeared to be present in all the glycolipids, but the concentration was very low in cerebrosides isolated from high density lipoproteins and erythrocytes, and in diglycosyl ceramide and globoside of the low density lipoproteins. In general, the average fatty acid chain length increased from cerebroside to globoside. This was most apparent in erythrocytes and also greater for normal acids than for hydroxy acids. Fatty acid distributions of erythrocyte glycolipids had sufficient variation to make a metabolic relationship by simple addition of a hexose appear doubtful. While the fatty acid distributions found in plasma lipoproteins were more similar, some means of acyl group selection is probably present for either the synthesis or degradation of these glycolipids.
Highlights
Four glycolipids have been isolated from three fractions of pig blood
The RBC were washed by resuspension in an Abbreviations: RBC, red blood cells; LDL, low density lipoprotein ;HDL, high density lipoprotein; GL-1, glycosyl ceramide; GL-2, diglycosyl ceramide; GL-3, triglycosyl ceramide; GL-4, globoside; GLC, gas-liquid chromatography; TLC, thin-layer chromatography
The cerebrosides (GL-1) from RBC of animal I1 were nearly twice those of I, while the trihexosides from RBC of pig I were twice those obtained from 11. These data are of the same order of magnitude as the results reported by Dawson and Sweeley [7] there was some variation in individual glycolipids
Summary
Four glycolipids have been isolated from three fractions of pig blood. The glycolipidswere presumably cerebroside, diglycosyl ceramide, triglycosyl ceramide, and globoside. The blood fractions were erythrocytes and plasma high and low density lipoproteins. Fatty acid distributions were determined for each glycolipid as a means to assist in identifying relationships among the several glycolipids. Hydroxy acids appeared to be present in all the glycolipids, but the concentration was very low in cerebrosides isolated from high density lipoproteins and erythrocytes, and in diglycosyl ceramide and globoside of the low density lipoproteins. The average fatty acid chain length increased from cerebroside to globoside. This was most apparent in erythrocytes and greater for normal acids than for hydroxy acids. While the fatty acid distributions found in plasma lipoproteins were more similar, some means of acyl group selection is probably present for either the synthesisor degradation of these glycolipids
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