Fate of a mutant emerging at the initial stage of evolution

Abstract

AbstractA simple system was constructed and used in the experimental elucidation of the fate of a mutant emerging in a population. Three Escherichia coli strains having the same genetic background except for their glutamine synthetase gene were used as model competitors. The difference in the enzyme gene were introduced by random mutation. Competition between these bacterial strains was carried out and observed in a continuous liquid culture. In most cases, the competitors stably coexist either in a steady state or in an oscillating state. In addition, the competition between the strains was found to be a deterministic process and not a stochastic one. These results showed that an emerging mutant in a population, be it a closely related one to the original members, can attain a state of stable coexistence even in a homogeneous environment. The ability of each of the emerging mutants to maintain its stable coexistence with the original population gives rise to the accumulation of various mutants in a population. Therefore, evolution starts from gradual accumulation of various mutants in the population, which in turn leads to the diversification of the population. As our experimental system is a minimum model for the various competitions in the natural ecosystem, the observed competitive coexistence is proposed to be a general phenomenon in nature.