Abstract
Ultra-performance liquid chromatographic technique was applied for the first time for determination of radiochemical and chemical purity of [11C]methionine with measurement time less than 2 min. The pH and eluent strength of mobile phase were optimized to achieve a gradient elution for separation of non-radioactive impurities as well as radioactive ingredients. The developed method was validated according to the following parameters: linearity, repeatability, recovery, limit of quantitation. The novel chromatographic procedure with reduced measurement time could be uniquely applied for the quality control of [11C]methionine to considerably shorten the release time and avoid radioactivity loss of radiopharmaceutical.
Highlights
Methionine has an important role in rapid synthesis of different proteins in brain tumors due to increased expression of amino acid transporters
It is well known from the literature that [11C]methionine injection may contain the following non–radioactive impurities: homocysteine, homocysteine thiolactone, homocystine, methionine and iodide ion [9, 13]
Homocysteine thiolactone can be only detected in the final product providing that it is used as a precursor for the radiosynthesis
Summary
Methionine has an important role in rapid synthesis of different proteins in brain tumors due to increased expression of amino acid transporters. Labeling the molecule with 11C isotope makes methionine a useful radiotracer for imaging oncological lesions with positron emission tomography (PET). Malignant tumor cell is characterized with high uptake of [11C]methionine. The accumulation of the radiolabeled amino acid in normal tissue is negligible. The extent of uptake is proportional with tumor grade. [11C]methionine is applied primarily in examination of gliomas to delineate uptake areas for surgery, radiotherapy and to guide biopsies [1]. The radiosynthesis of [11C]methionine is performed by methylation of L–homocysteine or L–homocysteine thiolactone with [ 11C]methyl iodide choosing various technical solutions.
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
