Abstract
A total dose of 7·65 × 10 5 erg cm −2 of far-red light (730 nm) was administered immediately after a total dose of 10 6 erg cm −2 of ultraviolet light (253 nm) treatment to mature dry pollen of Tradescantia paludosa . Three control groups were maintained: UV alone (UV), far-red alone (FR), and dark control (DC), in order to determine the effect of far-red light on UVinduced chromatid aberrations (UV+FR). Chromatid aberrations, including exchanges, chromatid and isochromatid breaks, were scored from metaphase figures of 18 hr old pollen tube culture grown on lactose agar medium supplemented with 0·02% colchicine. The average results of duplicated experiments indicated that the UV + FR group had about 46 per cent lower aberration rate than the UV group, while the FR and DC groups yielded similar background aberration rates. The lower rate of aberration in UV+FR group was postulated to be either the result of mitotic cessation for UV+FR induced aberrant cells which therefore failed to reach metaphase stage to be scored for aberrations, or far-red light enhanced reparation of UV-induced breakage.
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