Abstract
Factors influencing asexual sporulation in vitro were studied for three isolates of Phytophthora fragariae. Maximum numbers of zoosporangia were obtained when an 11 or 15 mm diam disc, cut at the edge of a 15- or 20-d-old colony on a semi-defined medium, was incubated in 6 ml sterile glass-distilled water, in a 4·5 cm diam Petri dish, at 13 or 22°C, depending on the isolate. Isolates varied in their response to the addition of nutrients to the flooding medium. Although visible light had no effect on asexual sporulation near-uv radiation inhibited zoosporangia production by two of the isolates. The role of nutrients as promoters and/or inhibitors of zoosporangia formation is discussed.
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