Abstract
The separation and purification of proteins is an essential precondition for proteomics research because of the intricate matrix environment. Hence, a facile method has been developed to synthesize hyperbranched polyethyleneimine modified magnetic nanomaterials (Fe3O4–NH2-BPEI) with dendritic structure, unique electrostatic effect, and abundant functional groups for the selective adsorption of proteins which greatly avoids the drawbacks of time consumption and leakage of metal ions in traditional pre-treatment. The preparation conditions, physical and chemical properties, and adsorption performance of Fe3O4–NH2-BPEI have been fully studied. The obtained materials have stable crystal shape, adequate superparamagnetism, fast adsorption kinetics, high adsorption amount, and admirable reusability. In addition, the as-prepared Fe3O4–NH2-BPEI exhibits good selective adsorption ability for electronegative proteins in the neutral solution, exhibiting a potential value for special adsorption for proteins with different pI.
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