Abstract
With the development of biomedical technology, personalized diagnosis and treatment at the single-cell level are becoming more important in the medical field. As one of the most powerful tools, microfluidic chips have shown significant potential for various applications related to cell separation, cell proliferation, and cell behavior analysis. However, fabricating microfluidic devices requires complicated procedures and high-cost equipment. In this study, an optofluidic maskless lithography method was proposed for rapid fabrication of microfluidic devices integrated with microwells. Through the use of this approach, microwells can be on-line designed and the exposure patterns can be modulated. Single or multi polystyrene microspheres were successfully trapped by using the designed microwells. The capture of MCF-7 cells and cell arrays indicated that the microfluidic devices fabricated in the present study can be applied for cell research.
Highlights
Owing to the developments in biological fields, personalized diagnoses and treatments at the single cell level have become important, which will introduce new potential breakthroughs for biomedicine [1,2,3,4]
We present optofluidic maskless lithography for rapid fabrication of microfluidic devices [23]
The optofluidic maskless lithography method was used to fabricate microfluidic chips integrated with microwell arrays
Summary
Owing to the developments in biological fields, personalized diagnoses and treatments at the single cell level have become important, which will introduce new potential breakthroughs for biomedicine [1,2,3,4]. Microfluidic device is a useful tool in cell research with advantages such as high-throughput manipulation, high level of integration, and requirement of less time and fewer reagents [5,6,7,8,9]. Cells trapped in a microfluidic device can be cultured, used to detect markers, manipulated, and employed for drug screening. Rettig et al have developed an easy-to-use method using microwell array fabricated through soft lithography for a single cell trap. Lin et al improved the traditional structure of microwells and introduced a double-well design, including a tapping well and a culturing well for single cell array construction. Human B cells infected by Kaposi’s sarcoma-associated herpesvirus (KSHV) were trapped in these microwells for 3D culture [14]
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