Abstract
In this work, a simple, rapid, and highly sensitive colorimetric assay for the determination of glutathione (GSH) was developed. It employs human serum albumin (HSA)-templated MnO2 nanosheets as an artificial oxidase. HSA-templated MnO2 nanosheets can oxidize 3,3′,5,5′-tetramethylbenzidine (TMB) to a blue oxTMB product with a significant increase in absorbance at 652 nm in the absence of H2O2. When GSH is introduced, the MnO2 nanosheets are reduced to Mn2+ ions, thereby inhibiting the formation of oxTMB. Based on these findings, a simple colorimetric assay was developed for the detection GSH in the range of 10 nM to 5 μM with a low detection limit of 5.6 nM. Importantly, the proposed method was successfully used for quantitative determination of GSH in biological fluids, such as human serum samples.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
