Fabrication of an ultrasensitive aptasensor for precise electrochemical detection of the trace amounts of streptomycin in milk

Abstract

Designing a sensitive method for the detection of streptomycin residues in animal products is essential for controlling consumer health risk. In this study, a high-purity pencil lead graphite electrode coated with inner graphene layers and outer surface-adsorbed gold nanoparticles attached to streptomycin-specific thiolated aptamer was used as an electrochemical aptasensor. The aptasensor electrode fabrication steps were investigated by scanning electron microscope (SEM) and Fourier-transform infrared spectrophotometer (FTIR). Moreover, aptasensor performance during fabrication and binding of aptamer to streptomycin were investigated by cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS) methods. After the binding of sreptomycin to it’s specific aptamer as a component of the aptasensor a decrease in the current and an increase in the charge transfer resistance (Rct) were recorded using the above-mentioned techniques. Under optimal conditions, the novel ultra-sensetive designed aptansensor detects streptomycin in the range of 10-8 to 10-16 M with a LOD of 0.8×10-18 M. The aptansensor demonstrates a high selectivity, good reproducibility and acceptable stability for the specific detection of streptomycin. According to the results, the manufactured aptansensor is a fast, low-cost, highly sensitive and selective device and thus the aptasensor can detect the trace amounts of streptomycin in milk in dairy industries.