Abstract
The objective of this research was twofold: first, to optimize the extraction process of Lonicera japonica polyphenols using a response surface methodology, and second, to study the antioxidant activity and tyrosinase inhibitory capacity of the polyphenols of different purities. High‐speed shearing homogenization extraction was used to extract the polyphenols from L. japonica. The antioxidant activity and the effect of polyphenols on tyrosinase activity were studied using free radical scavenging assay and the tyrosinase method, respectively. The optimal extraction conditions with an extraction yield of 6.96% for polyphenols were determined as follows: ethanol volume fraction 57%, shearing time 3.30 min, and solid–liquid ratio 1:58. Lonicera japonica polyphenols exhibited potent scavenging activity on 1,1‐diphenyl‐2‐picrylhydrazyl (DPPH) and 2, 2'‐azino‐bis(3‐ethylbenzothiazoline‐6‐sulfonic acid) (ABTS), and inhibitory capacity on tyrosinase. The results suggested that L. japonica polyphenols could be explored as a natural antioxidant and tyrosinase inhibitor.
Highlights
The herb, Lonicera japonica, is a member of the Caprifoliaceae family and is regularly used in traditional Chinese medicine
Recent studies have shown that extracts from L. japonica have a wide range of bioactive properties, such as anti‐inflammatory (Chen, Liou, Tzeng, Lee, & Liu, 2012), antibacterial (Xiong et al, 2013), and antioxidant use (Wang et al, 2014), and are used to treat nephrotic diabetes (Tzeng, Liou, Chang, & Liu, 2014) and metabolic diseases (Shin et al, 2017)
Tyrosinases (EC1.14.18.1), one of the extracts from L. japonica, are metalloenzymes belonging to the type‐3 copper protein (Rolff, Schottenheim, Decker, & Tuczek, 2011) that exist widely in microorganisms (Faccio, Kruus, Saloheimo, & Thony‐Meyer, 2012), animals (Hu, Wang, Deng, & Jiang, 2016), and plants (Zekiri et al, 2014)
Summary
The herb, Lonicera japonica, is a member of the Caprifoliaceae family and is regularly used in traditional Chinese medicine. Recent studies have shown that extracts from L. japonica have a wide range of bioactive properties, such as anti‐inflammatory (Chen, Liou, Tzeng, Lee, & Liu, 2012), antibacterial (Xiong et al, 2013), and antioxidant use (Wang et al, 2014), and are used to treat nephrotic diabetes (Tzeng, Liou, Chang, & Liu, 2014) and metabolic diseases (Shin et al, 2017). Tyrosinases (EC1.14.18.1), one of the extracts from L. japonica, are metalloenzymes belonging to the type‐3 (binuclear) copper protein (Rolff, Schottenheim, Decker, & Tuczek, 2011) that exist widely in microorganisms (Faccio, Kruus, Saloheimo, & Thony‐Meyer, 2012), animals (Hu, Wang, Deng, & Jiang, 2016), and plants (Zekiri et al, 2014). They are related to enzymatic browning in fruit and vegetables, pigmentation, and insect physiological processes (Balabanidou, Grigoraki, & Vontas, 2018; Olmedo et al, 2018; Xue et al, 2018)
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