Extraction of amylase from the microorganism isolated from textile mill effluent vis a vis desizing of cotton

Abstract

Amylase is one of the most popular enzymes in industrial biotechnology and is used widely in different industries such as food, detergent, textile, biofuel, and alcohol production. In textile wet processing, amylase is utilized for a preparatory process called desizing where it helps in the removal of starch from the grey cotton fabric. Even though a wide variety of products based on amylase are available in the market, the extraction of amylase from the novel source which can meet the requirements of desizing remains the quest of research. In the present work, textile mill effluent was utilized as a novel source for the isolation of fungus (Aspergillus), which can produce extracellular thermostable amylase. Temperature and pH optimization of amylase activity was studied, which reveals the broad range temperature stability of the extracted amylase. Zymogram analysis of this enzyme has shown the one isoform of amylase of approximately 55 kDa. The partially purified enzyme was applied on fabric and the treated fabric was analyzed for the efficiency of desizing. The extracted amylase displayed a good level of activity under the broad range of temperature and pH which can be considered as the ideal case for enzymatic desizing. The grey fabric was efficiently desized using the extracted amylase which was confirmed through TEGEWA ratings and absorbency of the desized fabric.