Abstract
This study was aimed to extract exopolysaccharide (EPS) produced by Listeria monocytogenes and investigate the maximum production by using different culture media and various factors. The EPS was obtained from the culture supernatant by cold ethanol precipitation and yield maximum production (0.368mg /ml ) in basal salt solution (BSS) as dry weight. The total carbohydrate content was determined by phenol sulfuric acid method. The functional groups were detected by Fourier transform infrared (FTIR) spectroscopy at frequency range of 400- 4000cm-1. Different alcohols were checked for their efficiency on precipitating EPS, those other than ethanol, methanol and isopropanol were able to sediment the EPS, while isoamylalcohol show no effect on precipitation.The results also indicated that the medium volume play an important role in EPS production.
Highlights
Listeria monocytogenes is a Gram-positive, facultative anaerobic, non-spore-forming, rodshaped bacterium with optimal growth temperature range of 30 - 37 ̊C
With a concern to find out the ability of L. monocytogenes to produce EPS in different chosen media, the quantity of EPS produced was determined and the results are shown in (Fig. 1), large amount of EPS production (0.368mg/ml) was obtained in BBS medium flowed by chemically defined medium (CDM) medium, the lowest EPS production was recorded with nitrogen- free medium (NFM) medium (0.102mg / ml) as dry weight
The results from the Fourier transform infrared (FTIR) spectroscopic analysis of EPS produced by the bacteria under study for the three tested media indicates the presence of different function groups such as C=O, COOH, and OH groups
Summary
Listeria monocytogenes is a Gram-positive, facultative anaerobic, non-spore-forming, rodshaped bacterium with optimal growth temperature range of 30 - 37 ̊C. It is one of the major foodborne pathogen causing disease in both humans and animals and has affected the food industries due to contamination of equipments that lead to cross contamination on food products (Chen et al, 2010; Pagadala et al, 2012). Attachment of pathogenic microbes leads to biofilm formation that will occurs when microcolony formed on the surface adheres with a conditioning film that traps the surrounding fluid and nutrients (Sauer et al, 2007; Lee et al, 2013).
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