Abstract
Various extractants have been used in determining plant available manganese in soil but critical comparisons among them are lacking. In our experiment, 14 extractants were tested using soil of three types (chernozem, pseudogley and humogley) from six locations. Neubauers bio test was used for evaluating capacity of extractants to determine plant-available Mn. Total Mn content in soil was in range 195–520 mg kg-1. Depending on the extractants used, 4–41% of total soil Mn was extractable. The lowest value of extractable Mn was obtained in CH3COONH4 (1 M) and the highest in EDTA (0.05 M). Statistically significant correlations between the biological test and chemical extraction were obtained for seven extractants tested: EDTA (0.05 M), HCl (1 M), H2SO4 (0.5 M), CH3COOH (0.4 M), H3PO4 (0.033 M), DTPA/CaCl2/TEA (0.005 M/0.1 M/0.1 M) and EDTA/(NH4)2CO3 (0.01 M/1 M). The most suitable extractants based on relationships between available soil Mn and Mn content in plants were 0.4 M CH3COOH and EDTA/(NH4)2CO3 for acid and alkaline soils, respectively.
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