Abstract
The human Periodontal Ligament Stem Cells (hPDLSCs) exhibit self-renewal capacity and clonogenicity potential. The Extracellular Vesicles (EVs) secreted by hPDLSCs are particles containing lipids, proteins, mRNAs, and non-coding RNAs, among which microRNAs, that are important in intercellular communication. The purpose of this study was the analysis of the non-coding RNAs contained in the EVs derived from hPDLSCs using Next Generation Sequencing. Moreover, our data were enriched using bioinformatic tools. The analysis highlighted the presence of non-coding RNAs and five microRNAs: MIR24-2, MIR142, MIR335, MIR490, and MIR296. Our results show that these miRNAs target the genes classified in two terms of the Gene Ontology: “Ras protein signal transduction” and “Actin/microtubule cytoskeleton organization.” Noteworthy, the in-deep analysis of our EVs highlights that the miRNAs could be implicated in the silencing of proto-oncogenes involved in 12 different types of tumors.
Highlights
The human Periodontal Ligament Stem Cells are mesenchymal stem cells that can be harvested from periodontal tissue
Using Human microRNA Disease Database (HMDD) (Huang et al, 2019) we reported in Table 1 the associations found among our miRNAs and the target genes deregulated in specific diseases
In the transcriptome of our Extracellular Vesicles (EVs), we found MIR490 that seems to have the role of tumor suppressor in lung cancer cells A549 and ovarian cancer by blocking the transcription of CCND1 and CDK1 respectively, two important genes involved in cell cycle progression (Gu et al, 2014; Chen et al, 2015)
Summary
The human Periodontal Ligament Stem Cells (hPDLSCs) are mesenchymal stem cells that can be harvested from periodontal tissue. The Extracellular Vesicles (EVs) are particles delimited by a lipid bilayer capable of crossing biological barriers and being internalized in the target cells so that they play an essential role. MicroRNAs in Extracellular Vesicles in intercellular communication (Valadi et al, 2007; Pizzicannella et al, 2019b). EVs contain different macromolecules such as lipids, proteins, DNA, mRNA and non-coding RNAs (ncRNAs) among which microRNAs (or miRNAs) (Zhang et al, 2019). The tapping-mode topographic 3D atomic force-microscopy measurements showed that EVs derived from hPDLSCs have a globular shape with a central depression and a relatively smooth surface (Diomede et al, 2018)
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