Abstract
Intestinal cell cultures offer unique possibilities to study the effect of extracellular matrix components on epithelial proliferation and differentiation. We have investigated the specific affinity for distinct matrix proteins, including laminin, collagen types I, III, and IV, and fibronectin, versus neutral control proteins in various small-intestine epithelial cell cultures. Both primary cells and intestinal epithelial cell lines display enhanced affinity for basement membrane constituents compared with interstitial collagens. Only the very undifferentiated, proliferative intestinal epithelial cells also synthesize these proteins, as determined by immunofluorescence and radioimmunoassay. On polarization and maturation, biosynthesis of basement membrane proteins is markedly reduced. Differentiation of intestinal epithelial cells is promoted only by laminin. Fibronectin and collagen type IV have no effect. Putative cell membrane receptors for individual basement membrane proteins are discussed.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callMore From: Scandinavian journal of gastroenterology. Supplement
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
