Abstract
BackgroundTo study on expressions and clinical significances of CD133 protein and CD133 mRNA in primary lesion of gastric adenocarcinoma (GC).MethodsExpressions of CD133 protein by immunostaining (99 cases) and CD133 mRNA by semi-quantitative RT-PCR (31 cases) were detected in primary lesion and in noncancerous gastric mucosa tissue (NCGT). Correlations of CD133 protein expression with clinicopathological parameters and post-operative survival were analyzed. Relations of CD133 mRNA level with Ki-67 labeling index (LI), and lymphatic metastasis were assessed too.ResultsBrown particles indicating CD133 protein positivity occurred in some parts of tumor cells and epithelium. Expressive percentage of CD133 protein positivity was significantly higher in subgroups with >5 cm diameter (P = 0.041), later TNM stage (P = 0.044), severer lymph node metastasis (P = 0.017), occurrences of lymphatic invasion (P = 0.000) and vascular invasion (P = 0.000) respectively. Severer invasion depth (P = 0.011), lymph node metastasis occurrence (P = 0.043) and later TNM stage (P = 0.049) were the independent risk factors for CD133 protein expression. Average brightness scale value (BSV) of CD133 mRNA was significantly higher in subgroups with >5 cm diameter (P = 0.041), lymph node metastasis occurrence (P = 0.004) and in lower Ki-67 LI (P = 0.02). Relative analysis revealed that BSV of CD133 mRNA related positively to metastatic lymphatic nodes ratio (P = 0.008) and metastatic lymph node number (P = 0.009), but negatively to Ki-67 LI (P = 0.009). Survival of positive subgroup of CD 133 protein was significantly poorer (P = 0.047). Lymph node metastasis occurrence (P = 0.042), later TNM stage (P = 0.046) and CD 133 protein positive expression (P = 0.046) were respectively the independent risk factors to survival.ConclusionHigher expressive level of CD133 mRNA is associated to lower Ki-67 LI and severer lymphatic metastasis. Therefore, the expressive level of CD133 mRNA can play an appropriate role to reflect the status of lymph node metastasis and proliferation of GC. CD133 protein expression is closely related with larger tumor, later TNM stage, lymphtic metastasis and survival of GC.
Highlights
To study on expressions and clinical significances of CD133 protein and CD133 mRNA in primary lesion of gastric adenocarcinoma (GC)
CD133 protein expression in primary lesion Particles sharing brown color indicated to CD133 protein expression occurred in some parts of gland parietes, cellular membrane surface of some tumor cells and some epithelium in primary lesion, in which CD133 positive particles mainly located in some parts of tumor cells in the mucosa and the submucosa layers (Figure 1C and 1D)
Correlation of CD133 protein expression with clinicopathological parameters CD133 expression was significantly correlated with tumor diameter of > 5 cm (P = 0.041), severer lymph node metastasis (P = 0.017), later TNM stage (P = 0.044), occurrences of lymphatic vessel infiltration (P = 0.000) and vascular infiltration (P = 0.000) (Table 1)
Summary
To study on expressions and clinical significances of CD133 protein and CD133 mRNA in primary lesion of gastric adenocarcinoma (GC). CD133 has been considered as a CSCs marker in many kinds of tumors such as colorectal [5,6], brain [4,7], prostate [8], pancreatic [11] and gastric cancers [12]. One of the aims in this study was to investigate the expression levels of CD133 protein and CD133 mRNA in primary lesion of gastric adenocarcinoma (GC) and to compare these expressive levels with clinicopathological characteristics and survival time after curative resection. At the same time, immunostaining for Ki-67, a kind of cellule nucleus protein, and its labeling index (LI) were applied to assess the proliferating ability of tumor cells with higher or lower CD133 mRNA level and the relation of this proliferating ability of tumor cells sharing higher or lower CD133 mRNA level were evaluated
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