Expression profile ofBMPR1Bgene in goat reproductive tract

Abstract

In the present work, the presence and distribution of BMPR1B transcripts in goat reproductive tract to elucidate its possible role in goat reproduction has been studies. Different samples of goat reproductive tract were collected, RNA was isolated and reverse transcribed with oligo (dT)18 primer by RT-PCR. BMPR1B and GAPDH gene expression was studied in all the samples by using PCR. Moreover, semi-quantitative analysis of BMPR1B gene expression in different tissues of Goat reproductive tract was performed using ImageJ software. RT-PCR displayed positive amplification of BMPR1B transcripts in small follicles, large follicles, corpus luteum, endometrium (pregnant and non pregnant) and placenta of goat by the amplification of 305 bp amplicon. With densitometric analysis, it was concluded that BMPR1B gene transcript level increases as the small follicles progresses to large follicular stage and falls to an intermediate level in corpus luteum, suggesting an important role of BMPR1B in ovulation and in luteogenesis (corpus luteum), which seems to be greatly accompanied by changes in the expression pattern of BMPR1B gene. Endometrial expression of this gene in cyclic and pregnant goats suggests that receptor may play role for the changes that take place in endometrium, required for normal uterine function such as successful fertilization, implantation and endometrial gland formation. The study clearly indicated that the normal endometrial/uterine cycle was accompanied by the significant BMP signaling and cycle specific expression of BMPR1B gene in goat reproductive tract.