Abstract
Cellulose is the world’s most abundant renewable energy resource, and a variety of cellulose synthase genes are involved in the biosynthesis of cellulose. In the process of cellulose synthesis, all cellulose synthases are interrelated and act synergistically. In this study, we analyzed the contents of cellulose, hemicellulose, and lignin in the different parts and tissues of E. grandis. The results showed that the cellulose content had greater differences among three different heights. On this basis, we carried out the transcriptome-wide profiling of gene expression patterns using RNA sequencing. A total of 2066 differentially expressed genes were identified for three pairwise comparisons between three different heights, most of which were related to the programmed photosynthetic membrane and photosystem. A total of 100 transcripts of CSs (58 CesA and 42 Csl) were obtained from transcriptome libraries. The expression pattern of these genes indicated that different CS genes had a wide range of expression profiles. A phylogenetic analysis of 135 reference CS genes showed that the CSs of E. grandis were clustered into six major groups (CesA1-9, CslA, CslB/H, CslD, CslE, and CslG). Based on the weighted gene co-expression network analysis, a dual-directional regulation mechanism between Csl and CesA proteins in the cellulose biosynthesis was identified. The gene expression profile analysis, using qRT-PCR in different tissues of E. grandis, demonstrated that the CSs were highly expressed in xylem, and CesAs had a higher relative expression than Csls. The analysis of sequence similarity combined with the expression pattern indicated that the CesA1, 3, and 6 transcripts were associated with the biosynthesis of the secondary cell wall, and CesA4, 5, and 7 transcripts were more likely to associate with the biosynthesis of the primary cell wall. Finally, the qRT-PCR analysis confirmed the expression of 11 selected CSs in three different parts of E. grandis.
Highlights
The plant cell wall is a cellular structure surrounding the outside of the plant protoplasm
This result is consistent with a previous study that found that lignin concentrations increased with the growth of wheat and maize [26]
The cellulose synthase A (CesA) formed different triplexes for the synthesis of primary and secondary cell or cellulose synthase-like (Csl) genes work together in E. grandis, we studied their coThe CesA formed for the synthesis of primary andrevealed secondary that cell NM_001302736.1 expression
Summary
The plant cell wall is a cellular structure surrounding the outside of the plant protoplasm It plays an important role in plant protection and morphogenesis, supplies water and nutrients, and maintains the interaction between the plant and the environment. The cell wall is considered as an important renewable plant biomass resource on earth. Cellulose is an important structural substance in cell walls and is the most abundant polysaccharide polymer in plants [1]. It plays a central role in plant development, and its biosynthesis is one of the most important biochemical processes in plant biology [2]. Nature produces approximately 1.8 × 1011 tons of cellulose every year [3], which is one of the most abundant biological macromolecules and an important renewable resource on earth [4]
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