Expression of the DMBT1 gene in breast cancer and its clinical significance

Abstract

Objective To investigate the relationship between mRNA expression of DMBT1 in breast cancer tissues and clinicopathological characteristics of patients with breast cancer.Methods 60 cases of breast cancer tissues,30 cases of breast paracancerous hyperplasia tissues and 30 cases of breast paracancerous normal tissues were selected,then reverse transcription-polymerase chain reaction (RT-PCR) were performed to detect the differential expression of DMBT1 in mRNA levels.Furthermore,its relationship with clinicopathological characteristics of patients with breast cancer was analyzed.Results 44 out of 60 breast cancer tissues were detected no or low expression of DMBT1 (73.3％),and 16 out of 60 cases were found normal expression of DMBT1(26.7％).There were statistically significant differences between paracancerous normal tissues and breast cancer tissues,also between paracancerous hyperplasia tissues and breast cancer tissues (x2 =11.72,P =0.000 62 ; x2 =15.99,P =0.000 06).No significant difference was found between the low expression of DMBT1 with the age of patients (x2 =1.733,P =0.188),the size of tumor (x2 =0.776,P =0.378) and the histological grade of tissues (x2 =1.000,P =0.316).However,the loss rates of DMBT1 mRNA expression in patients with lymph node metastasis and clinical stage Ⅲ were higher than that in patients without metastasis and clinicalⅠ-Ⅱstage (x2 =4.885,P =0.026 ; x2 =4.600,P =0.032).Conclusion Low expression of DMBT1 mRNA is closely associated with the metastasis and clinical stage of breast cancer. Key words: Breast neoplasms; DMBT1 gene