Abstract
The CCCTC - binding factor (CTCF) is a protein involved in repression, activation, hormone-inducible gene silencing, functional reading of imprinted genes and X-chromosome inactivation. We analyzed CTCF gene expression in bovine peripheral blood, oocytes and in different cellular stages (2-4 cells, 8-16 cells, 16-32 cells, morulae, and blastocysts) of in vitro fertilized embryos. This is the first report of CTCF expression in oocytes and preimplantation bovine embryos and has implications for the production of embryonic stem cells and the development of novel medical technologies for humans.
Highlights
The CCCTC - binding factor (CTCF) is a protein involved in repression, activation, hormone-inducible gene silencing, functional reading of imprinted genes and X-chromosome inactivation
The CTCF protein is involved with repression, activation and hormone-inducible gene silencing, creating constitutive chromatin insulators or boundaries and the functional reading of the imprinted genes (Rasko et al, 2001; Klenova et al, 2002)
The CTCF has been reported to interact with the imprinting control region (ICR) mapped in the region coding for human Prader-Willi/Angelman syndromes and a differentially methylated region (DMR) within the domain of reciprocally imprinted Dlk and Gtl2 genes (Klenova et al, 2002)
Summary
The CCCTC - binding factor (CTCF) is a protein involved in repression, activation, hormone-inducible gene silencing, functional reading of imprinted genes and X-chromosome inactivation. Epimutations in DMRs overlapping CTCF-binding sites have been showed in mouse preimplantation embryos cultured in different mediums (Doherty et al, 2000; Khosla et al, 2001).
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