Abstract
In response to an elicitor, the Ca 2+-dependent fluorescence (Fluo-3-Ca 2+) increased transiently and then the expression of the chitinase gene ( chi) followed. The gene expression was detected by Northern analysis. The deletion of Ca 2+ from the medium or the addition of a Ca 2+ channel blocker, verapamil, to the medium caused no gene expression, which supported the key role of Ca 2+ in the signaling towards the chi expression. Then the Ca 2+-injection experiment was done in order to investigate if it could trigger the chi expression. The plasmid pCHI-GFP (promoter: chi, reporter: green fluorescent protein gene ( gfp)) was injected into the single-protoplasts, then after 1 day of incubation at 25 °C, 100 μM CaCl 2 was injected into the same cells. After successive incubation for 1 day, 41 out of 85 cells showed the gene expression. The injection of 100 μM MgCl 2, however, caused no gene expression. Therefore, Ca 2+ could induce the chi of rice in the absence of the elicitor stimulus.
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