Abstract
A cDNA-derived 200 bp fragment encoding a rabbit defensin NP-1 mature peptide was ligated into the GUS-less pBH21 to yield pBIC-35NP1. Tobacco leaf discs were genetically transformed with anAgrobacterium tumefaclens strain harbouring pBIC-3SNP1. Northern blot analysis showed that the defensin NP-1 expression cassette was normally transcribed in transgenic plants. Resistance tests demonstrated that expression of native defensin NP-1 can confer partial resistance to the bacterial wilt pathogen,Ralstonia solanacearum.
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