Abstract
To investigate the effect of P2 receptor on microglia and its inhibitor PPADS on choroidal neovascularization. Forty CX3CR1GFP/+ mice were randomly divided into 8 groups. In addition to the normal group, the rest of groups were receiving laser treatment. The retina and choroid from the second, third, fourth and fifth group of mice were taken in the 1, 4, 7, 14 days after laser treatment. The mice in the sixth and seventh group received intravitreal injection of 2 µl PPADS or PBS respectively immediately after laser treatment. The mice in the eighth group received topical application of PPADS once per day of three days. The mice in sixth, seventh and eighth group received AF and FFA examination on the fourth day after laser treatment. Immunofluorescence histochemical staining and real-time quantitative PCR were used to evaluate P2 expression and its effect on choroidal neovascularization. After laser treatment, activated microglia can express P2 receptors (P2X4, P2X7, P2Y2 and P2Y12). The expression of P2 increased on the first day after laser damage, peaked on the fourth day (tP2X4 = 6.05, tP2X7 = 2.95, tP2Y2 = 3.67, tP2Y12 = 5.98, all P < 0.01), and then decreased. After PPADS inhibition, compared with the PBS injection group, the mRNA of P2X4, P2X7, P2Y2 and P2Y12 were decreased significantly in the PPADS injection group (tP2X4 = 5.54, tP2X7 = 9.82, tP2Y2 = 3.86, tP2Y12 = 7.91, all P < 0.01) and the PPADS topical application group (tP2X4 = 3.24, tP2X7 = 5.89, tP2Y2 = 6.75, tP2Y12 = 4.97, all P < 0.01). Compared with the PBS injection group, not only the activity of microglia cells but also the leakage of CNV decreased significantly (P < 0.01) in the PPADS injection group and the PPADS topical application group. But between two PPADS groups, the leakage of CNV had no difference (P = 0.864). After laser induced CNV, activated microglia can express P2 receptors. The P2 receptor inhibitor, PPADS, can significantly affect the function of microglia and inhibit the formation of choroidal neovascularization.
Highlights
To investigate the effect of P2 receptor on microglia and its inhibitor PPADS on choroidal neovascularization
Many questions still remain that what the exact function of microglia in pathogenesis of Choroidal neovascularisation (CNV) and how it changes its behaviour in the diseased retina compared to a healthy one
For the activated microglial cells in the laser spot, we studied whether there were P2 receptors expressed by microglial cells, and whether PPADS, a P2 antagonist, could influence the behaviour of microglia and have effect on the CNV
Summary
To investigate the effect of P2 receptor on microglia and its inhibitor PPADS on choroidal neovascularization. Compared with the PBS injection group, the activity of microglia cells and the leakage of CNV decreased significantly (P < 0.01) in the PPADS injection group and the PPADS topical application group. Choroidal neovascularisation (CNV) is the complication of many ocular fundus diseases, such as wet age-related macular degeneration (wAMD), pathologic myopia (PM), punctate inner choroidal angiopathy (PIC), choroidal rupture, etc. Some further investigations are still needed to confirm whether the inhibition of P2 receptors can influence the behaviour of microglial cells or change the pathological situation of CNV formation. For the activated microglial cells in the laser spot, we studied whether there were P2 receptors expressed by microglial cells, and whether PPADS (pyridoxalphosphate-6-azopheny 1-2’,4’-disulfonic acid), a P2 antagonist, could influence the behaviour of microglia and have effect on the CNV
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
