Abstract
Objective To investigate the relationship between the expression of microRNA (miRNA, miR)-4286 and the clinicopathological features in non-small cell lung cancer (NSCLC). Methods The expressions of miR-4286 in NSCLC tissue microarray and fresh tissue cryopreserved samples were detected by in situ hybridization and real-time quantitative polymerase chain reaction (Real-time PCR), respectively. The relationship between miR-4286 and clinicopathological features in NSCLC was analyzed. Results The positive expression rate of miR-4286 (62.14%, 87/140) was significantly higher than that of normal adjacent tissues (4.2%, 6/140; χ2=105.63, P 0.05). The results from Real-time PCR showed that miR-4286 expression level was highly observed in NSCLC tissues [0.032 (0.024-0.057)] when compared with that in normal adjacent tissues [0.027 (0.018-0.041), Z=-3.956, P<0.05]. Moreover, higher expression level of miR-4286 was correlated with TNM stage and lymph node metastasis (Z=-2.006, -2.016, P<0.05) in NSCLC. Conclusion The high expression of miR-4286 in NSCLC was associated with the disease progression and metastatic potential. Key words: Non-small cell lung cancer; MicroRNA-4286; In situ hybridization; Real-time quantitative polymerase chain reaction
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