Abstract
Objective To investigate the expression of microRNA (miRNA)-375 and its genomic promoter DNA methylation in esophageal squamous cell carcinoma (ESCC).Methods The expression of miRNA-375 in ESCC and the matched normal esophageal tissues (from the tumor edge ＞ 3 cm) was detected by real-time fluorescent quantitative polymerase chain reaction (FQ-PCR).DNA methylation status was examined by methylation specific polymerease chain reaction (MSP).The association between miRNA-375 expression and clinical factors were analyzed statistically.Results As compared with normal controls,miRNA-375 was significantly decreased in ESCC.The methylation levels in ESCC tissues were significantly higher than in the matched normal esophageal tissues (P ＜ 0.05).The low expression of miRNA-375 was significandy associated with ESCC TNM stage (P ＜ 0.05).Conclusion The lower expression of miRNA-375 and aberrant hypermethylation of the CpG island promoters in ESCC may play a crucial role in the carcinogenesis of ESCC. Key words: Esophageal squamous cell carcinoma; MicroRNA-375; DNA methylation
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