Abstract
Objective: To evaluate the pulp tissue of rat molars after pulpotomies with mineral trioxide aggregate (MTA), BiodentineTM (BDT) and calcium hydroxide (CH) mixed with sterile saline solution (24 hours, 72 hours, 7 days and 15 days), through correlating MPO activity with active neutrophils and MMP8 activity with tissue remodeling. Material and Methods: Thirty-eight Wistar rats were randomly distributed into groups (control, I (MTA gray), II (BDT), and III (CH)) and subdivided according to the study period of 24 hours, 72 hours, 7 days or 15 days after pulpotomy. MMP8 activity was assessed through fluorescence technique, and MPO activity was determined using the MPO assay. Results: A gradual decrease of MPO and MM8 activity occurred in the group MTA over the experimental periods (p<0.05). Groups BDT and CH exhibited an increase in the activity at 7 and 15 days (p<0.05). Conclusion: MTA demonstrated a decrease in the values of MPO e MMP8. BDT and CH showed high neutrophilic and collagenase activity over the experimental periods.
Highlights
After pulpotomy, a biocompatible and bioactive material should protect the remaining vital pulp [1-4]
mineral trioxide aggregate (MTA) demonstrated a decrease in the values of MPO e Matrix Metalloproteinase-8 (MMP8)
Calcium hydroxide (CH), Mineral trioxide aggregate (MTA) and, more recently, BiodentineTM (BDT) are biomaterials indicated for pulpotomy [1,2,3,5]
Summary
A biocompatible and bioactive material should protect the remaining vital pulp [1-4]. A biocompatible and bioactive material should protect the remaining vital pulp [1-. Calcium hydroxide (CH), Mineral trioxide aggregate (MTA) and, more recently, BiodentineTM (BDT) are biomaterials indicated for pulpotomy [1,2,3,5]. MTA exhibits faster pulp tissue healing and formation of mineralized tissue [3,5,6,7]. A disadvantage of MTA includes the high cost [8,9]. CH has demonstrated the formation of dentin bridge, but it was irregular [3,10,11]. Limited areas of coagulative necrosis are commonly seen after CH use [7,12]. BiodentineTM (BDT) shows higher mechanical and handling properties than MTA [10,13]
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