Expression of human protection of telomere 1 and its relationship with Helicobacter pylori infection in gastric carcinoma

Abstract

Objective To determine the expression of human protection of telomeres 1 (hPOT1) and its relationship with Helicobacter pylori (HP) infection in gastric carcinoma. Methods The expressions of hPOT1 protein and hPOT mRNA were detected in 53 gastric carcinoma specimens (observed group) and 20 normal gastric mucosa tissues (control group) by SP immunohistochemical method and in situ hybridization (ISH), respectively. HP infection was examined by Warthin-Starry method in observed group. Results The positive expression rate of hPOT1 protein was 84.91 % (45/53) in observed group, higher than that in control group [30.00 % (6/20)] (P<0.01). The positive expression rate of hPOT1 mRNA was 58.49 % (31/53) in observed group, higher than that in control group [10.00 % (2/20)] (P<0.05). The positive co-expression rate of hPOT1 protein and mRNA was 56.60 % (30/53), both had positive relationship in gastric carcinoma (r=0.394, P<0.05). The rate of HP infection in 53 cases of gastric carcinoma was 52.83 % (28/53). The positive expression rates of hPOT1 protein and mRNA in observed group with HP infection were significantly higher than those in observed group without HP infection[protein: 96.43 % (27/28) vs 72.00 % (18/25), P<0.05; mRNA: 85.75 % (24/28) vs 28.00 % (7/25), P<0.01]. Conclusions hPOT1 may be associated with occurrence of gastric carcinoma. Combined detection of hPOT1 protein and mRNA can be used for the diagnosis of gastric carcinoma. HP infection may be associated with abnormal expression of hPOT1 in occurrence of gastric carcinoma. Key words: Stomach neoplasms; Human protection of telomeres 1; Helicobacter pylori