Expression of human Biot2 and its potential function on carcinogenesis in endometrial cancer

Abstract

Rat Biot2 is a novel gene first reported and named by our laboratory. We wanted to study the expression profile and reveal the function of its human homologous gene (human Biot2, hBiot2) on carcinogenesis in endometrial cancer. Using RT-PCR, we tested hBiot2 expression profile in normal human tissues from infants and adults. Using real-time RT-PCR, we tested different expression quantities of hBiot2 between an endometrial cancer group and a normal endometrium group, between different histological grading groups in endometrial cancer, and between different stage groups during the menstrual cycle. RNA-RNA ISH tested the expression location of hBiot2 in normal and cancer tissues. Three kinds of novel shearings of hBiot2 in different normal tissues were obtained. Normal organs expressing hBiot2 in infants, did not express hBiot2 in adults. hBiot2 expression was higher in endometrial cancer tissue compared to normal endometrium tissue. hBiot2 expression was higher in the Grade 2-3 group with endometrial cancer compared to the Grade 1 group, and it was higher in the proliferative phase than in the secretory phase of a normal endometrium. hBiot2 was expressed mainly in the parenchymal cells. Three shearings of hBiot2 may suggest that it has different functions in different organs. The expression difference of hBiot2 between infants and adults, cancerous and normal tissues, different histological gradings in endometrial cancer, different stages during the menstrual cycle in the normal endometrium suggests that hBiot2 may have the potential to induce carcinogenesis in endometrial cancer.