Expression of CYP2W1 in gastric cancer and its clinical significance

Abstract

Objective To investigate the CYP2W1 expression and its clinical significance in gastric cancer. Methods The expressions of CYP2W1 protein in 326 adenocarcinoma of gastric structures and in the corresponding 326 normal gastric mucosa structures were tested by immunohistochemical technique. The expressions of CYP2W1 mRNA in 10 random chosen examples of adenocarcinoma of gastric structures and its corresponding normal gastric mucosa structures were tested by semi-quantitative RT-PCR. The association of CYP2W1 expression with clinicopathologic features and prognosis in gastric cancer patients were analyzed with various statistical methods, such as χ2-test, Kaplan-Meier method and COX proportional hazard model. Results CYP2W1 protein and CYP2W1 mRNA in the gastric cancer structure clearly was more than that in normal gastric mucosa structure(P<0.05). The gastric cancer patient’s survival rate with high expression of CYP2W1 was dramatically lower than that with low expression of CYP2W1. The expression of CYP2W1 was closely correlated with stomach cancer patient’s prognosis(χ2=7.699, P=0.006). The result of COX proportional hazard model showed that CYP2W1 was the independent prognostic factor of stomach cancer patient(P=0.019). Conclusions CYP2W1 only expresses in the gastric cancer structure. However, it doesn’t express in the normal gastric mucosa structure; The expression of CYP2W1 is closely correlated with the prognosis; CYP2W1 is the independent prognostic factor of gastric cancer. Key words: Gastric cancer; CYP2W1; Clinicopathologic features; Prognosis