Expression of CD24 as Cancer Stem Cell Marker in the Diagnosis of Oral Squamous Cell Carcinoma - A Prospective Study.

Abstract

Oral squamous cell carcinoma (OSCC) is an aggressive and recurrent malignancy. Identification of unique and overexpressed cell surface antigens is important in the diagnosis and development of cancer vaccines and various therapies for OSCC. We have used real-time polymerase chain reaction (RT-PCR) for the expression of cell surface protein CD24 in both tissue samples and in blood samples to study the clinicopathological features as well as to determine the gene expression profile of CD24 in OSCC and explore its role as a potential target of clinical therapy. In this prospective study, the expression of CD24 was evaluated in 20 blood (3 ml) and tissue samples of OSCC specimens by quantitative RT-PCR. Student's t-test was used for statistical analysis. The significance level was considered <0.05. CD24 was found to be upregulated amongst the cases for both the tissue and the blood. CD24 was statistically significant with P < 0.05. Fold change was calculated to assess the quantity of the difference in expression amongst cases when compared to controls. Results were supportive of CD24 being a reliable biomarker, hence blood samples can also be used in screening and diagnosis of OSCC. CD24 expression is significantly upregulated in blood and tissue samples in OSCC. In addition, CD24 overexpression is highly associated with adverse prognostic parameters such as lymph node involvement, advanced clinical stages and worse overall survival. Our findings have important implications in future practice, overexpression of CD24 in OSCC was associated with poor prognosis correlating to the clinical findings, large-scale comprehensive studies are needed further to confirm our findings. In addition to histological features, CD24 can be used as marker for OSCC.