Abstract
Purpose: To study the levels of reactive oxygen species (ROS), nuclear transcription factor-κBp65 (NF-κBp65) and TGF-1, and their correlation in bronchopulmonary dysplasia in neonatal rats.Methods: Twenty (20) pregnant rats were randomly divided into study and normal groups. Radial alveolar counts were carried out at 2, 8 and 15 days of age. The levels of ROS expression in the lung tissues of the two groups were assayed by ELISA while immunohistochemistry was used to determine the expressions of TGF-β1 and NF-κBp65 in neonatal lung tissues of the two groups. Pearson correlation test was used to analyze correlations amongst ROS, TGF-β1 and NF-κBp65 in the neonatal lung tissues.Results: At days 8 and 9 after birth, radial alveolar count was significantly lower in study rats than in control rats (p < 0.05). Expression levels of ROS, TGF-β1 and NF-κBp65 in study group were markedly raised at days 2, 4 and 8 after birth, relative to control (p < 0.05).Conclusion: The levels of ROS, TGF-β1 and NF-κBp65 in bronchopulmonary dysplasia in neonatal rats are significantly and positively correlated, and are higher than those in normal rats. This provides a scientific basis for development of drugs for bronchopulmonary dysplasia.
 Keywords: Neonatal rats, Bronchopulmonary dysplasia, Lung tissue, NF-κBp65, TGF-β1, Correlation
Highlights
Bronchopulmonary dysplasia, a chronic pulmonary disease in premature infants, is characterized by alveolar and pulmonary microvascular dysplasia due to inflammation [1]
Studies have found that Reactive oxygen species (ROS), TGF-β1and nuclear transcription factor-κBp65 (NFκBp65) are closely associated with the etiology of bronchopulmonary dysplasia
ROS, TGF-β1 and NF-κBp65 levels were significantly increased in the study group at days 2, 4 and 8 after birth, relative to normal rats, and the expression levels of ROS, NF-κBp65 and TGF-β1 in study group were significantly increased at the three time points
Summary
Bronchopulmonary dysplasia, a chronic pulmonary disease in premature infants, is characterized by alveolar and pulmonary microvascular dysplasia due to inflammation [1]. A new type of bronchopulmonary dysplasia with complicated pathogenesis refers to stunted or stagnant lung development in premature babies Research suggest that this new type of bronchopulmonary dysplasia is the result of a combination of multiple factors [2]. Lung tissues of the neonates in both groups were excised and weighed. Body weight and lung weight of neonatal rats in the two groups were recorded and compared. Following H&E staining, lung tissue histomorphological changes in the two groups of neonatal rats were examined using light microscopy. Radiative alveolar counts were monitored at three time points after birth: days 2, 4 and 8 At each of these time points, small sections of lung tissue were taken, cut into smaller sections, and homogenized. The correlations amongst ROS, NF-κBp65 and TGF-β1 expression in lung tissues of newborn rats were analyzed with Pearson correlation test. Results of statistical comparisons were considered significant at p < 0.05
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
