Abstract
To investigate the expression and distribution of junctional adhesion molecule 1 (JAM-1) in human corneal tissue and cells. Reverse transcriptase-polymerase chain reaction was used to detect the expression of JAM-1, ZO-1, and occludin mRNAs in corneal cells, while the presence of JAM-1 protein was analyzed by flow cytometry (FACS). Double immunofluorescence staining was used to determine the tissue distribution of JAM-1 and occludin in human corneas. Strong expression of JAM-1, ZO-1, and occludin mRNAs was observed in primary cultured corneal epithelial and endothelial cells, but not in primary cultured keratocytes. The expression of JAM-1 protein in cultured epithelial and endothelial cells was confirmed by FACS. When keratocytes were cultured in medium with 10% fetal calf serum for several passages, differentiation into corneal myofibroblasts occurred. The expression of JAM-1 was detected in these corneal myofibroblasts at both RNA and protein levels. JAM-1 immunoreactivity was seen at cell borders throughout the entire epithelium, but not in keratocytes from normal corneal tissue. On the other hand, JAM-1 immunoreactivity was detected in the cytoplasm of corneal endothelial cells. JAM-1 is expressed by human corneal epithelial and endothelial cells, but not by keratocytes, although its expression is induced in corneal myofibroblasts.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.