Abstract
Background. Aberrant expression of microRNA-146a (miR-146a) has been found in several classes of cancers. However, its expression and clinicopathological contribution in hepatocellular carcinoma (HCC) has not been fully elucidated.Objective. To explore the clinicopathological significance of the miR-146a level in HCC formalin-fixed paraffin-embedded (FFPE) tissue.Methods. Eighty-five HCC samples and their para-cancerous normal liver tissues were collected. Total mRNA including miRNA was extracted, and miR-146a expression was determined using real-time RT-PCR. Furthermore, the correlation between the miR-146a expression and clinicopathological parameters was investigated.Results. MicroRNA-146a expression in HCC tissues was lower compared with that in adjacent non-cancerous hepatic tissues. MicroRNA-146a expression was also related to clinical TNM stage, metastasis, portal vein tumor embolus, and number of tumor nodes.Conclusions. Down-regulation of miR-146a is related to HCC carcinogenesis and deterioration of HCC. MicroRNA-146a may act as a suppressor miRNA of HCC, and it is therefore a potential prognostic biomarker for HCC patients.
Highlights
Primary hepatocellular carcinoma (HCC) is the third leading cause of death from cancers, with an estimated 549,000 deaths per year, which ranks it as the fifth most frequent malignancy worldwide [1,2,3,4,5]
We investigated the expression of miRNA-146a in HCC and their matched adjacent non-cancerous liver tissues in formalin-fixed paraffin-embedded (FFPE) surgically resected samples
Lower levels of miR-146a were found in HCC patients with metastasis, portal vein tumor embolus, and multiple tumor nodes, compared with patients without corresponding traits
Summary
Primary hepatocellular carcinoma (HCC) is the third leading cause of death from cancers, with an estimated 549,000 deaths per year, which ranks it as the fifth most frequent malignancy worldwide [1,2,3,4,5]. The carcinogenesis and progression of HCC are typical of a multistage process. New biomarkers are urgently required to understand factors causing hepatocarcinogenesis and to link diverse phenotypes in clinical features and prognosis. Aberrant expression of microRNA-146a (miR-146a) has been found in several classes of cancers. Its expression and clinicopathological contribution in hepatocellular carcinoma (HCC) has not been fully elucidated. To explore the clinicopathological significance of the miR-146a level in HCC formalin-fixed paraffin-embedded (FFPE) tissue. The correlation between the miR-146a expression and clinicopathological parameters was investigated. MicroRNA-146a expression in HCC tissues was lower compared with that in adjacent non-cancerous hepatic tissues. MicroRNA-146a expression was related to clinical TNM stage, metastasis, portal vein tumor embolus, and number of tumor nodes. MicroRNA-146a may act as a suppressor miRNA of HCC, and it is a potential prognostic biomarker for HCC patients
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