Abstract
Juvenile Hormone (JH) modulates a variety of developmental and physiological processes in Insects. Previous studies suggest that the two hydrolytic enzymes namely Juvenile Hormone Esterase (JHE) and Juvenile Hormone Epoxide Hydrolase (JHEH) play important roles in the regulation of JH titer. Compared with JHE, JHEH is more essential regulatory enzyme and could be applied to control insects, because of its irreversible reaction on JH Mechanism. A full length of cDNA (1323bp) encoding juvenile hormone epoxide hydrolase gene from Spilarctia obliqua (SoJHEH) is isolated and subcloned into baculovirus vector system using pFastBac and pFastBac-HTA vectors. The bacmid-transformed SoJHEH is expressed in Insect cell line system and the expression of recombinant SoJHEH is confirmed by Western blotting. The enzyme activity of recombinant SoJHEH showed a high activity with JH III and very low with other general epoxide substrates. The deduced SoJHEH protein sequence showed the conserved domains of catalytic triad and oxyanion hole indicating the well preserved catalytic mechanism of epoxide hydration which can be explored further for bio control strategy at molecular level using insect’s own enzyme which can address the current problem of insect resistance.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
