Abstract
The characteristics of the transport system for inorganic phosphate (Pi) have been investigated in chicken osteoclasts. Pi transport in these cells was mainly Nadependent and also energy-dependent, suggesting the existence of Na-Pi cotransport system in these cells. While the time course of Pi transport in chicken osteoclasts was comparable to that in chicken mononuclear cells, the precursors of the osteoclasts, Pi transport was stimulated in a time- and dose-dependent fashion, when the osteoclasts were exposed to bone particles. The stimulation was not due tode novo synthesis of Pi carrier, since the stimulatory effect was very rapid with the maximal effect observed at 1 h and could not be blocked by 50 µM cycloheximide. On the other hand, inhibition of osteoclast attachment to bone particles by adding an excess of Arg-Gly-Asp-Ser peptide blocked the stimulation. In summary, stimulation of Pi transport in chicken osteoclasts was induced by bone particles. Attachment of the osteoclasts to bone particles with integrin receptors appeared to be crucial to this stimulation.
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