Abstract
The impact of nanotechnology in the field of agricultural sciences creates the need to study in greater detail the effect of products offering nanoparticles for application in plant species of agricultural interest. The objective of this study was to determine the response of stevia (Stevia rebaudiana B.) in vitro to different concentrations of AgNPs (silver nanoparticles), as well as to characterize and identify their absorption, translocation and accumulation mechanisms. Nodal segments of stevia grown in MS medium supplemented with AgNPs (0,12.5, 25, 50,100 and 200 mg L−1) were used. After 30 days of in vitro shoot proliferation, the number of shoots per explant, shoot length, chlorophyll content, dry matter content and the metallic silver (Ag) content of the plants were quantified. In addition, characterization, transport and accumulation of silver nanoparticles were performed by microscopic analysis. AgNPs were shown to be present in epidermal stem cells, within vascular bundles and in intermembrane spaces. In leaves, they were observed in ribs and stomata. The current and future use of AgNPs in agricultural sciences opens up the possibility of studying their effects on different plant species.
Highlights
The use of silver nanoparticles (AgNPs) in biological systems is part of the development of bionanotechnology
In vitro culture is a technique that allows studying the behavior of AgNPs in plants of agricultural interest such as stevia (Stevia rebaudiana B.)
It is possible to find more studies on the location of AgNPs in roots[16,19] in this study we focus on locating AgNPs in stem and leaf tissues of stevia in vitro using fluorescence microscopy techniques
Summary
The use of silver nanoparticles (AgNPs) in biological systems is part of the development of bionanotechnology This discipline offers alternatives to solve problems related to the production of food crops by inhibiting microbial agents[1,2,3]. The development of new methods to characterize and identify AgNPs in cells and tissues would contribute to a better understanding of the potential effects during in vitro culture of plant tissues. In vitro culture is a technique that allows studying the behavior of AgNPs in plants of agricultural interest such as stevia (Stevia rebaudiana B.). The objective of this study was to characterize the physicochemical properties of AgNPs formulated as Agrovit, as well as to study the effect of their concentration on the development of stevia (Stevia rebaudiana B.) grown in vitro. Properties Metallic silver content (% wt.) PVP Content (% wt.) Form Factor (Spheroid) Average diameter of metallic silver particles by TEM data (nm) Round Size interval of metallic silver particles by TEM (nm) Zeta potential (mV) Fluorescence in violet, green and red (nm)
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
