Exposure of red spruce seedlings to acid mist: importance of droplet composition just prior to drying periods.

Abstract

Two-year-old red spruce (Picea rubens Sarg.), raised from seed collected at five locations from Maine to North Carolina, were exposed in a greenhouse to either sulphuric or nitric acid mist (median pH 2-85). Exposures were performed three times a day, four days a week over an eleven-week period during the growing season. Each exposure consisted of 4 h of misting followed by a 3.75 h drying period. Immediately before each drying period, acidic droplets were either left to dry directly on the foliage or were removed by a 15 min deionized water (diw) rinse. When sulphuric acid droplets dried directly on the foliage, visible injury developed and to a lesser degree the same was true for nitric acid. When acidic droplets were replaced with dim before each drying period, foliar injury did not develop despite misting for hundreds of hours at pH values well within the range that has caused visible injury in previous experiments. When acidic droplets dried directly on foliage, needle dry weight was reduced, but differences between sulphuric and nitric acid exposure were not found. No treatment effects were found on terminal shoot length. Treatment responses among seedlings from different seed resources were similar, with greater variation found within provenances than between provenances. We conclude that the composition of liquid on foliage at the end of wet deposition events and the processes occurring during drying periods are important factors in determining the response of red spruce to acidic cloudwater.