Exploring the anti-Helicobacter pylori activity and mechanism of Shouhui Tongbian through chemical composition analysis and network pharmacology.

Effects of Helicobacter pylori and Nonsteroidal Anti-Inflammatory Drugs on Peptic Ulcer Disease: A Systematic Review

The aim of the preset study was to determine the efficacy of Annona Senegalensis stem and root extracts against Salmonella typhimurium Shigella flexneri and Escherichia coli through the evaluation of bacterial sensitivity and determination of the minimum inhibitory and bactericidal concentration of the extracts against the test isolates. Plant materials were collected and duly authenticated. The methanoic and aqueous extracts prepared from the powdered forms were tested on the bacterial after cultural and biochemical identification of the isolates. The antibiotic sensitivity test was carried out using the Kirby Bauer disk diffusion method while chloramphenicol was used as the standard control. The Minimum Inhibitory Concentration (MIC) of the plant extracts were determined by broth dilution method while the Minimum Bactericidal Concentration (MBC) was determined by a method described using standard protocols. The ratio of MBC:MIC was computed to determine the bactericidal or bacteriostatic effects of the extracts. Data were analyzed using the Minitab 16 statistical package. Descriptive statistics (mean and standard error) and analysis of variance tools were applied while mean separation was done Fischer’s method at 5% level of significance. Antibacterial sensitivity test showed that the control test (Chloramphenicol) had significantly higher antibacterial sensitivity ( P<0.05) than any of the plant extract. Minimum Inhibitory Concentration (MIC) of the plant extract ranged from 6.25 mg/ml to 25.0 mg/ml. The lowest MIC of 6.25mg/ml was observed in Salmonella typhimurium among all extract types. Root and stem had similar effects on the test organisms (P>0.05) but, methanoic root extract had the lowest MBC of 6.25mg/ml against S.typhimurium and S. flexneri. Based on the MBC/MIC ratio, all extract types had bactericidal effects on Salmonella typhimurium and Shigella flexneri except aqueous root extract that showed bacteriostatic effect. Only the methanoic root and stem extracts exhibited bactericidal effects on Escherichia coli. Annona Senegalensis root and stem could possibly be explored commercially as an antibacterial agent against species of Salmonella, Shigellia and Escherichia.

Effective eradication therapy for Helicobacter pylori is a worldwide demand. Aspartate α-decarboxylase (ADC) was reported as a drug target in H. pylori, in an in silico study, with malonic acid (MA) as its inhibitor. We evaluated eradicating H. pylori infection through ADC inhibition and the possibility of resistance development. MA binding to ADC was modeled via molecular docking. The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of MA were determined against H. pylori ATCC 43504, and a clinical H. pylori isolate. To confirm selective ADC inhibition, we redetermined the MIC in the presence of products of the inhibited enzymatic pathway: β-alanine and pantothenate. HPLC was used to assay the enzymatic activity of H. pylori 6x-his tagged ADC in the presence of different MA concentrations. H. pylori strains were serially exposed to MA for 14 passages, and the MICs were determined. Cytotoxicity in different cell lines was tested. The efficiency of ADC inhibition in treating H. pylori infections was evaluated using a Sprague–Dawley (SD) rat infection model. MA spectrum of activity was determined in different pathogens. MA binds to H. pylori ADC active site with a good docking score. The MIC of MA against H. pylori ranged from 0.5 to 0.75 mg/mL with MBC of 1.5 mg/mL. Increasing β-alanine and pantothenate concentrations proportionally increased MA MIC. The 6x-his tagged ADC activity decreased by increasing MA concentration. No resistance to ADC inhibition was recorded after 14 passages; MA lacked cytotoxicity in all tested cell lines. ADC inhibition effectively eradicated H. pylori infection in SD rats. MA had MIC between 0.625 to 1.25 mg/mL against the tested bacterial pathogens. In conclusion, ADC is a promising target for effectively eradicating H. pylori infection that is not affected by resistance development, besides being of broad-spectrum presence in different pathogens. MA provides a lead molecule for the development of an anti-helicobacter ADC inhibitor. This provides hope for saving the lives of those at high risk of infection with the carcinogenic H. pylori.

Identification of potential therapeutic targets and epithelial cell signaling pathway for Helicobacter pylori infection using biosurfactants as a novel green antimicrobials: A network pharmacology approach.

BackgroundHelicobacter pylori (H. pylori) infection has become an international public health problem, and antibiotic-based triple or quadruple therapy is currently the mainstay of treatment. However, the effectiveness of these therapies decreases due to resistance to multiple commonly used antibiotics. Sanguisorba officinalis L. (S. officinalis), a traditional Chinese medicine clinically used for hemostasis and treatment of diarrhea, has various pharmacological activities. In this study, in vitro antimicrobial activity was used for the preliminary evaluation of S. officinalis against H. pylori. And a pharmacology analysis approach was also utilized to elucidate its underlying mechanisms against H. pylori infection.MethodsMicro-broth dilution method, agar dilution method, checkerboard assay, scanning electron microscopy (SEM), and transmission electron microscopy (TEM) were used for the assessment of anti-bacterial activity. Active ingredients screening, GO analysis, KEGG analysis, construction of PPI network, molecular docking, and RT-qPCR were used to elucidate the underlying pharmacological mechanisms of S. officinalis against H. pylori infection.ResultsThe minimum inhibitory concentration (MIC) values of S. officinalis against multiple H. pylori strains including clinically isolated multi-drug resistant (MDR) strains were ranging from 160 to 320 µg/ml. These results showed that S. officinalis had additive interaction with four commonly used antibiotics and could exert antibacterial effect by changing the morphology of bacteria without developing drug resistance. Through network pharmacology analysis, 8 active ingredients in S. officinalis were screened out for subsequent studies. Among 222 putative targets of S. officinalis, 49 targets were identified as potential targets for treatment of H. pylori infection. And these 49 targets were significantly enriched in GO processes such as protein kinase B signaling, protein kinase activity, protein kinase binding, and KEGG pathways such as Pathways in cancer, MicroRNAs in cancer, and TNF signaling pathway. Protein-protein interaction analysis yielded 5 core targets (AKT1, VEGFA, EGFR, SRC, CCND1), which were validated by molecular docking and RT-qPCR.ConclusionsOverall, this study confirmed the in vitro inhibitory activity of S. officinalis against H. pylori and explored the possible pharmacological mechanisms, laying the foundation for further research and clinical application.

To evaluate the accuracy of several methods aimed to detect Helicobacter pylori stool antigens in patients with upper gastrointestinal bleeding. Thirty-four patients with upper gastrointestinal bleeding because of peptic ulcer were included. The first stool sample during hospitalization was collected, and stool antigens were determined with: polyclonal enzyme-linked immunosorbent assay (Premier-Platinum-HpSA); monoclonal enzyme-linked immunosorbent assay (Amplified-IDEIA-HpStAR); and rapid monoclonal immunochromatographic test (ImmunoCard-STAT HpSA). A patient was considered infected when H. pylori was diagnosed with invasive tests (rapid urease test or histology) or with (13)C-urea breath test. When all tests were negative, a new breath test was repeated after stopping proton pump inhibitors. All patients were infected and, therefore, only sensitivity of the tests could be calculated: polyclonal enzyme-linked immunosorbent assay (74%), monoclonal enzyme-linked immunosorbent assay (94%), and rapid monoclonal immunochromatographic test (60%; concordance between the two observers was high, kappa = 0.9). Neither the presence of maelena nor the delay in obtaining stool samples explained false negatives. Neither the polyclonal enzyme-linked immunosorbent assay stool antigen test nor the rapid immunochromatographic stool antigen test can be recommended to diagnose H. pylori infection in patients with upper gastrointestinal bleeding. However, the monoclonal enzyme-linked immunosorbent assay stool antigen test is highly sensitive for detecting the infection in patients with this complication, although more studies are necessary to evaluate the specificity of the method.

Further data on the association between Helicobacter pylori infection and primary open-angle glaucoma

Prevalence of Helicobacter pylori resistant strains in the southern part of Switzerland

In vitro antibacterial activity of nimbolide against Helicobacter pylori

The present study evaluates the antimicrobial activity of fractionated extracts ofAgeratum conyzoides in a bid to isolate the active constituents of the plant with anti-Helicobacter pylori activity. Helicobacter pylori was isolated from the specimens following standard microbiology procedures and isolates subjected to pure fractions of plant extracts for antimicrobial assays. Extracts of A. conyzoides was fractionated by silica gel and thin layer chromatography to obtain pure fractions (17). Fractions 23 - 30 and 31 - 36 were so close and had crystals; it was assumed that they had the same active components, so they were combined and considered as one (Fractions 23 - 36). The disk diffusion method was used to determine the susceptibility of 15 strains of H. pylori to the fractions. The Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) for the most active fraction was also determined by the broth dilution method. Results were analyzed by the Fisher’s exact test. All the fractions tested demonstrated antimicrobial activity with zone diameters of inhibition between 0 – 30 mm. However, two of the 17 fractions [23 - 36(100%Hex-Hex/EA20%) and 69 - 83(Hex/EA80%)] demonstrated very potent activities. The lowest MIC and MBC recorded were 0.002 and 0.016 mg/mL respectively. However the MIC of the fractions ranged from 0.016 - 0.500 mg/mL for fractions 23 - 36 and 0.002 - 0.500 mg/mL for fractions 69 - 83. The MBC of the fractions ranged from 0.063 - 0.500 mg/mL for fractions 23 - 36; 0.016 - 1.000 mg/mL for fractions 69 - 83. There was a statistically significant difference (P 0.05) in their activities both for the MIC and MBC. It is concluded that this plant may contain compounds with therapeutic activity, which may be found in fractions 23 - 36 (100%Hex-Hex/EA20%) and 69 - 83(Hex/EA80%). Key words: Antimicrobial activity, medicinal plant, minimum inhibitory concentration, minimum bactericidal concentration, Helicobacter pylori, antimicrobial resistance.

Helicobacter pylori (H. pylori) infection triggers a sequence of gastric alterations starting with an inflammation of the gastric mucosa that, in some cases, evolves to gastric cancer. Efficient vaccination has not been achieved, thus it is essential to find alternative therapies, particularly in the nutritional field. The current study evaluated whether curcumin could attenuate inflammation of the gastric mucosa due to H. pylori infection. Twenty-eight C57BL/6 mice, were inoculated with the H. pylori SS1 strain; ten non-infected mice were used as controls. H. pylori infection in live mice was followed-up using a modified 13C-Urea Breath Test (13C-UBT) and quantitative real-time polymerase chain reaction (PCR). Histologically confirmed, gastritis was observed in 42% of infected non-treated mice at both 6 and 18 weeks post-infection. These mice showed an up-regulation of the expression of inflammatory cytokines and chemokines, as well as of toll-like receptors (TLRs) and MyD88, at both time points. Treatment with curcumin decreased the expression of all these mediators. No inflammation was observed by histology in this group. Curcumin treatment exerted a significant anti-inflammatory effect in H. pylori-infected mucosa, pointing to the promising role of a nutritional approach in the prevention of H. pylori induced deleterious inflammation while the eradication or prevention of colonization by effective vaccine is not available.

The frequency of Helicobacter pylori (HP) infection in 208 patients with upper gastrointestinal tract symptoms from the Southern Province of Saudi Arabia was studied prospectively. The occurrence of HP was documented histologically and using a rapid urease test in antral endoscopic biopsies. Our results showed that 82.2% of the 208 patients included were positive for HP with a male:female ratio of approximately 1:1 (88:83). The age range was 14 to 80 years and the median age was 38.2 years. The frequencies of HP infection among Saudi and non-Saudi patients were 86% and 71%, respectively. Frequencies of HP infection were 88%, 77.5%, and 93% during the second, third, and fourth decades of life. Among the 140 patients with histologically proven antral gastritis, 128 cases (91%) were positive for HP whereas 29 cases (17%) of the 171 patients positive for HP did not show histologic evidence of antral gastritis. Our data showed that HP was present in 92.5% of patients with endoscopic diagnosis of duodenal ulceration, 81% of patients with duodenitis, 80% of patients with both duodenitis and gastritis, 69% of patients with gastric antral erythema, and 81% of patients with non-ulcer dyspepsia (normal upper gastrointestinal endoscopy). Histologically proven antral gastritis was seen in 80% of patients with endoscopic diagnosis of duodenal ulceration, 76% of patients with antral erythema, 70% of patients with both duodenitis and gastritis, 33% of patients with duodenitis only, and 66% of patients with non-ulcer dyspepsia. Among the 208 patients included in the study, gastric ulcerationw as only seen in two cases, both positive for HP.

Potential mechanisms by which Jiawei Lianpu Yin inhibits Helicobacter pylori colonization and alleviates gastric mucosal inflammation and damage: Integrated transcriptomics, network pharmacology, and experimental validation.

The Prevalence of Idiopathic Peptic Ulcer and Changing Trend of Peptic Ulcer Disease and Helicobacter Pylori Infection Between 10 Years in Korea

Variants in Autophagy Genes Affect Susceptibility to Both Crohn's Disease and Helicobacter pylori Infection