Abstract
Study was aimed to screen the adult female of different buffalo breeds maintained at organized herd of ICAR-NDRI, Karnal (Haryana) and from field (Punjab, Himachal Pradesh and Chhattisgarh state) for the presence or absence of important genetic variations in 3’UTR region of Signal Transducer and Activator of Transcription 1 (STAT1) gene, known to be associated with mammary gland development and with milk fat% and milk yield in cows. Animals from three breeds of riverine buffaloes Murrah, Gojri and Chhattisgarhi were studied using PCR-RFLP technique with HaeIII restriction enzyme. It revealed monomorphic patterns in the 3’UTR region STAT1 gene studied in all the three breeds of riverine buffaloes, indicating absence of genetic variation at this loci and suggesting that the loci is fixed for buffaloes in contract to exotic cattle breeds.
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