Abstract
Leucanthemum paludosum is a small, popular, perennial herbaceous flower that is widely used as a potted plant and in flower beds. It is highly adaptable and fast-growing, blooms from early to late spring, and can be maintained for two–three months, thus making it a potential model plant for cytological studies. However, it lacks applicable markers for identifying its chromosome numbers, and no karyotyping or cytological study about L. paludosum has been reported. In the present study, we aimed to analyze the morphology and chromosomal status of L. paludosum to provide a reference for its systematic utilization. The results showed that L. paludosum is diploid (2n = 2x = 18). The bivalent number was substantially higher than the monovalent bodies. L. paludosum also conducted a loosely patterned karyotype, with no fluorescence in situ hybridization (FISH) signal when using a conventional oligonucleotide (oligo) probe library. To address this problem, we developed a novel repetitive oligo probe ((ATC)10) using transcriptome sequences. Furthermore, we explored its feasibility in different plants and provided effective repetitive oligoprobes for species such as Avena sativa, Vicia faba, Allium sativum, Lilium davidii var. unicolor, and Antirrhinum majus. Our study provides new cytological data for L. paludosum and developed a novel method for designing oligo probes for karyotype detection.
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