Experimental transmission of Enteromyxum leei Diamant, Lom and Dykova, 1994 in sharpsnout sea bream, Diplodus puntazzo C. and the effect on some innate immune parameters

Abstract

Enteromyxum leei has been implicated in outbreaks in sharpsnout sea bream, causing a serious economic impact on Mediterranean fish farming. Information about transmission and life cycles of marine myxosporea is limited to only a few fish species. In the present study, direct transmission of the infection was achieved by cohabitation of infected and healthy Diplodus puntazzo C. for the first time. The progress of the infection was monitored by the detection of different developmental stages of the parasite in the posterior intestine. Infected fish with E. leei were firstly observed in day 5 (prevalence of 20%). The total prevalence reached 100% in day 19 and was maintained in this high level until the end of the experimental period (day 55). In addition, the initial intensity of the different parasite stages was low, but on day 26 the intensity of trophozoites was suddenly increased, followed by the increased intensity of sporoblasts and mature spores. Also, the experimental transmission of E. leei in recovered D. puntazzo previously treated for parasite elimination proved to be unsuccessful, suggesting of a possible development of protective immunity against the parasite.