Experimental study on the expression and significance of high mobility group protein B1 in breast cancer

Abstract

Objective To investigate the high mobility group protein B1 (HMGB1) expression and significance in breast cancer.Methods (1) Collecting breast cancer tissues and paracancerous tissues from 20 breast cancer cases who underwent surgery in January 2014 in the First Affiliated Hospital of Fujian Medical University,using reverse transcriptase-polymerase chain reaction (RT-PCR) methods to detect the expression of HMGB1 gene.(2) Selecting breast cancer tissue and paracancerous tissue paraffin block specimens from 100 patients with different breast cancer stages in The Breast Cancer Database of the hospital from January 2012 to December 2012 according to the time sequence,and using immunohistochemistry methods to detect the expression of HMGB1 protein,then analyzing the relationship between the expression of HMGB1 and clinical pathological characteristics of breast cancer.Results Compared to paracancerous tissues,HMGB1 ' s gene expression rate and the amount of protein expression are both higher in breast cancer tissues,respectively,15.94 ± 1.42 vs.1.29 ± 0.41,and 66％ vs.18％ (P ＜ 0.01) ; the expression of HMGB1 protein is closely related to the extent of lymph node metastasis and tissue grading (P ＜0.05),but has no relation with age,family history of malignant tumor or tumor diameter (P ＞0.05).Conclusion The abnormal expression of HMGB1 gene and protein level have important guiding significance for early diagnosis and analysis on prognosis of breast cancer,and it may has a good application prospect to develop treatment on its target spot. Key words: Breast cancer; High mobility group protein B1 ; Reverse transcriptase-polymerase chain reaction ; Immunohistochemistry