Experimental Study On Implications of Rev-1 Vaccine Against Brucella melitensis Infection In The Dog

Abstract

Background: Canine brucellosis may occur due to Brucella spp. other than Brucella canis. Dogs transmit Brucella among other dogs, ruminants and human. So there is a need for using vaccine for hosts of Brucella especially dogs. Objectives: The present manuscript describes the efficacy of Rev.1 against Brucella melitensis in experimentally infected dogs. Methods: Twelve Brucella negative dogs were divided into the test and control groups. The animals in the experimental group were vaccinated with Rev.1. After vaccination, sera of the dogs were tested by standard tube agglutination test and Rose Bengal test. Five months after vaccination dogs in the both groups were innoculated with 3×109 CFU of Brucella melitensis biotype 1. Serum samples were taken after inoculation of the bacterium and were examined using Rose Bengal test and standard tube agglutination test. Specimens of lymph nodes and reticuloendothelial organs were collected for bacteriological culture. Results: After inoculation of Brucella, the antibody titer was significantly higher in the control dogs than in the experimental group. Brucella melitensis biotype 1 was isolated from all the control dogs but it was isolated from 3 dogs in the experimental group. Conclusions: Based on this study, we recommend that additional studies should be performed to be able to say conclusively that along with vaccination of small ruminants, dogs be immunized with Rev.1 vaccine.