Abstract
Second harmonic generation (SHG) is a well-known physical phenomenon of important in nonlinear optics, which has been widely used in tissue morphology and pathology. In this thesis, the influences on backward SHG intensity were studied. A two-photon laser scanning confocal microscope coupled with a mode-locked femtosecond Ti: sapphire laser was used to carry out the experiments. Rat-tails were used as experimental objects, there were 40 slices including transverse and longitudinal. All slices were un-dyed. The changes of backward SHG intensities of rat-tails versus the excited wavelengths, excited powers, exploring-depths and so on were obtained. It was clear that different irradiation conditions had evident influences on backward SHG intensity. Subsequently, the backward SHG imaging and two-photon excited fluorescence (TPEF) imaging through two independent channels were also compared preliminarily. This study demonstrates that SHG is a promising tool for more accurate information in biomedicine fields.
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