Abstract
An experimental study of the influence of the conformational change suffered by molecular beacon (MB) probes-upon the biorecognition of nucleic acid target oligonucleotides over evanescent wave photonic sensors-is reported. To this end, high sensitivity photonic sensors based on silicon photonic bandgap (PBG) structures were used, where the MB probes were immobilized via their 5' termination. Those MBs incorporate a biotin moiety close to their 3' termination in order to selectively bind a streptavidin molecule to them. The different photonic sensing responses obtained toward the target oligonucleotide detection, when the streptavidin molecule was bound to the MB probes or not, demonstrate the conformational change suffered by the MB upon hybridization, which promotes the displacement of the streptavidin molecule away from the surface of the photonic sensing structure.
Highlights
The development of new strategies for detecting DNA and RNA strands has become highly interesting for several application fields such as food quality control [1, 2], pathogen detection [3, 4] or biological safety [5]
We report an experimental demonstration of the influence of the conformational change of molecule-labeled molecular beacon (MB) probes over an evanescent wave photonic sensing structure
We have experimentally observed the influence of the conformational changes of MB probes over evanescent wave photonic sensing structures
Summary
The development of new strategies for detecting DNA and RNA strands has become highly interesting for several application fields such as food quality control [1, 2], pathogen detection [3, 4] or biological safety [5]. A particle/molecule, able to interact with an electrochemical transducer, is attached to the MB probe, so that a variation in the measured electrical signal is produced when that particle/molecule is displaced due to the conformational change of the MB after hybridization [28,29,30,31] Those works demonstrate the high potential of exploiting the hairpin conformational changes of MBs upon hybridization changing the interaction of a given molecule/particle with a transduction element, that can open the door to the development of high performance MB-based biosensing devices. Within this context, we report an experimental demonstration of the influence of the conformational change of molecule-labeled MB probes over an evanescent wave photonic sensing structure. That response of the photonic sensor having streptavidinlabeled MB probes will be compared with the case when streptavidin-free MB probes are used in order to determine the influence of the displacement of the molecule on the optical sensing response
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
