Abstract
This paper presents optimization of matrix-assisted laser desorption/ionization (MALDI) time-of-flight (TOF) mass spectrometer (MS) instrumental parameters for the analysis of chloro(2,2'',2"-terpyridine)palladium(II) chloride dihydrate complex applying design of experiments methodology (DoE). This complex is of interest for potential use in the cancer therapy. DoE methodology was proved to succeed in optimization of many complex analytical problems. However, it has been poorly used for MALDI-TOF-MS optimization up to now. The theoretical mathematical relationships which explain the influence of important experimental factors (laser energy, grid voltage and number of laser shots) on the selected responses (signal to noise ? S/N ratio and the resolution ? R of the leading peak) is established. The optimal instrumental settings providing maximal S/N and R are identified and experimentally verified.
Highlights
This paper presents optimization of matrix-assisted laser desorption/ionization (MALDI) time-of-flight (TOF) mass spectrometer (MS) instrumental parameters for the analysis of chloro(2,2',2"-terpyridine)palladium(II) chloride dihydrate complex applying design of experiments methodology (DoE)
The DoE is used for the optimization of instrumental parameters of equipment for analysis at graphite furnace atomic absorption spectrometer (GF AAS) [15,16], inductively coupled plasma optical emission spectrometer (ICP OES) [17,18], inductively coupled plasma mass spectrometer (ICP-MS) [19], and matrix-assisted laser desorption/ionization time-of-flight mass spectrometer (MALDI-TOF-MS) [1,20,21,22,23,24,25]
Two responses are selected as relevant for the quality of the obtained spectra: signal to noise ratio (S/N signifies the level of a desired signal to the level of background noise) and resolution (R represents the ability of a mass spectrometer to distinguish between the ions of different m/z ratios)
Summary
This paper presents optimization of matrix-assisted laser desorption/ionization (MALDI) time-of-flight (TOF) mass spectrometer (MS) instrumental parameters for the analysis of chloro(2,2',2"-terpyridine)palladium(II) chloride dihydrate complex applying design of experiments methodology (DoE). The DoE is used for the optimization of instrumental parameters of equipment for analysis at graphite furnace atomic absorption spectrometer (GF AAS) [15,16], inductively coupled plasma optical emission spectrometer (ICP OES) [17,18], inductively coupled plasma mass spectrometer (ICP-MS) [19], and matrix-assisted laser desorption/ionization time-of-flight mass spectrometer (MALDI-TOF-MS) [1,20,21,22,23,24,25] This methodology involves performing of small number of carefully planned experiments, which allows the establishment of mathematical relationship between investigated factors and important system responses.
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