Examining the Influence of Spatial Smoothing on Spatiotemporal Features of Intrinsic Connectivity Networks at Low ICA Model Order.

Abstract

Using a relatively high model order of independent component analysis (ICA with 75 ICs) of functional magnetic resonance imaging (fMRI) data, we have reported a clear effect of spatial smoothing Gaussian kernel size on spatiotemporal properties of intrinsic connectivity networks (ICNs). However, many if not the majority of ICA fMRI studies are usually performed at low model order, e.g., 20-IC decomposition, as such low order is generally enough to extract the few networks of interest such as the default-mode network (DMN). The aim of this study is to investigate if we can replicate the spatial smoothing effects on spatiotemporal features of ICNs at low ICA model order. Same resting state fMRI data that we used with 75-IC analysis were used here. Spatial smoothing using an isotropic Gaussian filter kernel with full width at half maximum (FWHM) of 4, 8, and 12 mm was applied during preprocessing. ICNs were identified from 20-IC decomposition and evaluated in terms of three primary features: spatial map intensity, functional network connectivity (FNC), and power spectra. The results identified similar effects of spatial smoothing on spatial map intensities and power spectra at p < 0.01, false discovery rate (FDR) corrected for multiple comparisons. Reduced spatial smoothing kernel size resulted in decreased spatial map intensities as well as a generally decreased low-frequency power (0.01 - 0.10 Hz) but increased high-frequency power (0.15 - 0.25 Hz). FNC, however, did not show a uniform change in correlation values with the size of smoothing kernel. Notably, FNC between DMNs decreased but FNC between central executive and visual networks increased with an increase in smoothing kernel size. These preliminary findings confirm spatial smoothing influences ICN features regardless of model order. The discussion focuses on differences between observed changes at low and high ICA model orders.