Abstract
BackgroundThe plant-specific Teosinte branched1/Cycloidea/Proliferating cell factor (TCP) family of transcription factors is involved in the regulation of cell growth and proliferation, performing diverse functions in plant growth and development. In addition, TCP transcription factors have recently been shown to be targets of pathogenic effectors and are likely to play a vital role in plant immunity. No comprehensive analysis of the TCP family members in potato (Solanum tuberosum L.) has been undertaken, however, and whether their functions are conserved in potato remains unknown.ResultsTo assess TCP gene evolution in potato, we identified TCP-like genes in several publicly available databases. A total of 23 non-redundant TCP transcription factor-encoding genes were identified in the potato genome and subsequently subjected to a systematic analysis that included determination of their phylogenetic relationships, gene structures and expression profiles in different potato tissues under basal conditions and after hormone treatments. These assays also confirmed the function of the class I TCP StTCP23 in the regulation of plant growth and defence.ConclusionsThis is the first genome-wide study including a systematic analysis of the StTCP gene family in potato. Identification of the possible functions of StTCPs in potato growth and defence provides valuable information for our understanding of the classification and functions of the TCP genes in potato.
Highlights
The plant-specific Teosinte branched1/Cycloidea/Proliferating cell factor (TCP) family of transcription factors is involved in the regulation of cell growth and proliferation, performing diverse functions in plant growth and development
Identification of TCP genes in Solanum tuberosum A total of 24 Arabidopsis and 20 S. tuberosum-specific TCP full-length amino acid sequences were recovered from the Arabidopsis Information Resource (TAIR) and Potato Genome Sequencing Consortium (PGSC) databases, respectively
Several Arabidopsis TCP genes have more than one counterpart in the potato genome, possibly as a result of the FPKM values and the results of the Quantitative reverse transcription polymerase chain reaction (qRT-PCR) analysis differed slightly, these analyses revealed that members of each clade exhibited similar expression patterns
Summary
The plant-specific Teosinte branched1/Cycloidea/Proliferating cell factor (TCP) family of transcription factors is involved in the regulation of cell growth and proliferation, performing diverse functions in plant growth and development. TCP transcription factors are characterized by the presence of a nonstandard basic helix-loop-helix (bHLH) motif of 59 amino acids responsible for DNA binding and protein-protein interactions [17, 18]. This domain was first identified from comparisons of four proteins that play key roles in plant morphological evolution and development: TEOSINTE BRANCHED 1 (TB1) in Zea mays, CYCLOIDEA (CYC) in Antirrhinum majus, and PROLIFERATING CELL FACTORS 1 and 2 (PCF1 and PCF2) in Oryza sativa [17, 19]. Class I is known as the PCF subclade; class II can be further subdivided into the CIN and the CYC/TB1 subclades [20]
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